产品编号:A153
产品描述:Anti-Human AIF(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human Apopotosis Inducing Factor (AIF)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: A153
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Apopotosis Inducing Factor (NT) (AIF) recognizes and epitope near the N-terminus of Human AIF. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins.1 A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF).2 Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli.2,3 AIF is highly conserved between human and mouse and widely expressed.2
Known Species Cross Reactivity: Hu+
Immunogen
AIF (NT) Blocking Peptide (Leinco Prod. No.: A172)
Blocking Peptide
AIF (NT) Blocking Peptide (Leinco Prod. No.: A172)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human AIF by Western blot analysis at a concentration of .25-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
References
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
2. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6
3. Daugas E, Susin SA, Zamzami N, et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J 2000;14:729-39 (WD0500)
Related Products
AIF (NT) Blocking Peptide (Leinco Prod. No.: A172)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Anti-Human Apoptosis Inducing Factor (Intermediate Domain) (AIF) (Leinco Prod. No.: A152)
Anti-Human Apoptosis Inducing Factor (CT) (AIF) (Leinco Prod. No.: A151)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
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