产品编号:A156
产品描述:Anti-Human Apaf-1(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human Apoptosis Protease Activating Factor-1 (Apaf-1)
Purified No Carrier Protein
(CED4)
Polyclonal Antibody
Prod. No.: A156
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Apoptosis Protease Activating Factor-1 (Apaf-1) recognizes an epitope near the C-terminus of human, mouse and rat Apaf-1. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1.1,2 Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis.3 Apaf1 can also associate with caspase-4 and caspase-8.4 Apaf1 transcript is ubiquitously expressed in human tissues.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Apoptosis Protease Activating Factor-1 (CT) Blocking Peptide (Leinco Prod. No.: A173)
Blocking Peptide
Apoptosis Protease Activating Factor-1 (CT) Blocking Peptide (Leinco Prod. No.: A173)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human Apaf-1 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Heart Tissue Lysate (Leinco Prod. No.: H1405).
References
1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (WD1299)
Related Products
Apoptosis Protease Activating Factor-1 (CT) Blocking Peptide (Leinco Prod. No.: A173)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Heart Tissue Lysate (Leinco Prod. No.: H1405)
Apoptosis Protease Activating Factor-1 (NT) Blocking Peptide (Leinco Prod. No.: A174)
Anti-Human Apoptosis Protease Activating Factor-1 (NT) (Apaf-1) (Leinco Prod. No.: A157)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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