产品编号:A159
产品描述:Anti-Human ARC(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human Apoptosis Repressor with CARD Domain (ARC)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: A159
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Apoptosis Repressor with CARD Domain (ARC) recognizes an epitope near the C-terminus of Human ARC. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD.1 ARC interacts with caspase-2 and –8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.1
Known Species Cross Reactivity: Hu+
Immunogen
Anti-Human Apoptosis Repressor with CARD Domain (CT) Blocking Peptide (Leinco Prod. No.: A177)
Blocking Peptide
Anti-Human Apoptosis Repressor with CARD Domain (CT) Blocking Peptide (Leinco Prod. No.: A177)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human ARC by Western blot analysis at a dilution of 1:1,000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are A549 Whole Cell Lysate (Human Lung Carcinoma) (Leinco Prod. No.: A181) or HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).
References
1. Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-60 (RD1299)
Related Products
Anti-Human Apoptosis Repressor with CARD Domain (CT) Blocking Peptide (Leinco Prod. No.: A177)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A549 Whole Cell Lysate (Human Lung Carcinoma) (Leinco Prod. No.: A181)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
Anti-Human Apoptosis Repressor with CARD Domain (NT) (ARC) (Leinco Prod. No.: A160)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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