产品编号:A249
产品描述:Anti-Human ARMER(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human ARMER
Purified No Carrier Protein
(ARL6IP, AIP1)
Polyclonal Antibody
Prod. No.: A249
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human ARMER recognizes an epitope near the C-terminus of human and mouse ARMER. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is important for normal development and tissue homeostasis. It is mediated by various caspases and ultimately results in the activation of endogenous endonucleases that degrade cellular DNA.1 Although apoptosis induced by endoplasmic reticulum (ER) stress is thought to be mediated by caspase-12,2 other caspases such as caspase-9 are also thought to be activated following ER stress.3 Recently, ARMER, a novel integral ER-membrane protein was shown to protect cells from ER stress-induced apoptosis. Analysis of the caspase proteolytic cascade suggests that ARMER acts by inhibiting caspase-9 activity,3 although the mechanism for this remains unkown. It should be noted that ARMER is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
ARMER (CT) Blocking Peptide (Leinco Prod. No.: A263)
Blocking Peptide
ARMER (CT) Blocking Peptide (Leinco Prod. No.: A263)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human and mouse ARMER by Western blot analysis at a concentration of 0.5-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Bladder Tissue Lysate (Leinco Prod. No.: B432).
References
1. Stellar H. Mechanisms and genes of cellular suicide. Science 1995; 267:1445-9.
2. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J. Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 2000; 403:98-103.
3. Lui HM, Chen J, Wang L, et al. ARMER, Apoptotic regulator in the membrane of the endoplasmic reticulum, a novel inhibitor of apoptosis. Mol. Cancer Res. 2003; 1:508-18.
Related Products
ARMER (CT) Blocking Peptide (Leinco Prod. No.: A263)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Bladder Tissue Lysate (Leinco Prod. No.: B432)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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