产品编号:A247
产品描述:Anti-Human APG7(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Autophagy protein 7 (APG7)
Purified No Carrier Protein
(ATG7)
Polyclonal Antibody
Prod. No.: A247
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Autophagy protein 7 (CT) (APG7) recognizes an epitope near the C-terminus of human and mouse APG7. This polyclonal antibody was purified using affinity chromatography.
Background:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components.1,2 This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1.3 Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog.4 In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles.5 It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.6
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Autophagy Protein 7 (CT) Blocking Peptide (Leinco Prod. No.: A259)
Blocking Peptide
Autophagy Protein 7 (CT) Blocking Peptide (Leinco Prod. No.: A259)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human APG7 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Caco-2 Whole Cell Lysate (Leinco Prod. No.: C1182).
References
1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4. Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.
Related Products
Autophagy Protein 7 (CT) Blocking Peptide (Leinco Prod. No.: A259)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Caco-2 Whole Cell Lysate (Leinco Prod. No.: C1182)
Anti-Human Autophagy protein 7 (NT) (APG7) (Leinco Prod. No.: A248)
Autophagy Protein 7 (NT) Blocking Peptides (Leinco Prod. No.: A260)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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