产品编号:B456
产品描述:Anti-Human BID
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human BID
Purified No Carrier Protein
(Caspase-8-Cleaved)
Polyclonal Antibody
Prod. No.: B456
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human BID recognizes an epitope in the intermediate domain of human and mouse Bid. This polyclonal antibody was purified using affinity chromatography.
Background:
Bid is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bid is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells Bid is cytosolic. In response to Fas ligand or TNF Bid is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane. Cleavage of Bid by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target Bid to the mitochondria. Bid may then interact with another pro-apoptotic Bcl-2 family member Bak. Interaction of Bid with Bak causes altered mitochondrial membrane permeability. A 9 - 13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the Bid interaction with other Bcl-2 family members. Bid is neutralized by binding to the anti-apoptotic member Bcl-x.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
BID (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B487)
Blocking Peptide
BID (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B487)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
3. Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
4. Wang K, Yin XM, Chao DT, et al. BID: a novel BH3 domain-only death agonist. Genes Dev. 1996; 10:2859-69.
Related Products
BID (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B487)
Anti-Human BID (CT) (Leinco Prod. No.: B455)
BID (CT) Blocking Peptide (Leinco Prod. No.: B486)
Recombinant Mouse BID (Leinco Prod. No.: B554)
Anti-Mouse BID (Leinco Prod. No.: B594)
Anti-Human/Mouse BID-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: B617)
Recombinant Human BID (Leinco Prod. No.: B550)
Recombinant Human BID (Leinco Prod. No.: B553)
Recombinant Mouse BID (Leinco Prod. No.: B549)
Anti-Human BID ( Anitgen Aff. Pur. ) (Leinco Prod. No.: B644)
Anti-Human/Mouse BID ( Antigen Aff. Pur. ) (Leinco Prod. No.: B645)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
.jpg)
