产品编号:B457
产品描述:Anti-Human Bif
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Bif
Purified No Carrier Protein
(Bax-interacting factor 1, endophilin B1)
Polyclonal Antibody
Prod. No.: B457
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Bif recognizes human and mouse Bif. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes.. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Bif Blocking Peptide (Leinco Prod. No.: B488)
Blocking Peptide
Bif Blocking Peptide (Leinco Prod. No.: B488)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect mouse and human Bif by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).
References
1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
2. Oltvai ZN, Milliman CL, and Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993; 74:609-19.
3. Cuddeback SM, Yamaguchi H, Komatsu K, et al. Molecular cloning and characterization of bif-1. J. Biol. Chem. 2001; 276:20559-65.
4. Takahashi Y, Karbowski M, Yamaguchi H, et al. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis. Mol. Cell. Biol. 2005; 25:9369-82.
Related Products
Bif Blocking Peptide (Leinco Prod. No.: B488)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
