产品编号:B462
产品描述:Anti-Human Bit1(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Bit1
Purified No Carrier Protein
(Bcl-2 inhibitor of transcription 1, Peptidyl-tRNA hydrolase 2)
Polyclonal Antibody
Prod. No.: B462
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Bit1 recognizes an epitope near the N-terminus of human, mouse and rat Bit1. This polyclonal antibody was purified using affinity chromatography.
Background:
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment.1 Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process.2 Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination.3 It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE.2 Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the α5β1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the α5β1 integrin.2
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Bit 1 (NT) Blocking Peptide (Leinco Prod. No.: B493)
Blocking Peptide
Bit 1 (NT) Blocking Peptide (Leinco Prod. No.: B493)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human, mouse and rat Bit1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is A-20 Whole Cell Lysate (Leinco Prod. No.: A232).
References
1. Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
Related Products
Bit 1 (NT) Blocking Peptide (Leinco Prod. No.: B493)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A-20 Whole Cell Lysate (Leinco Prod. No.: A232)
Anti-Human Bit1 (Intermediate Domain) (Leinco Prod. No.: B461)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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