产品编号:B481
产品描述:Anti-Human BMI-1
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human BMI-1
Purified No Carrier Protein
(Polycomb Group RING Finger Protein 4, RNF51 )
Polyclonal Antibody
Prod. No.: B481
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human BMI-1 recognizes an epitope in the intermediate domain of human, mouse and rat BMI-1. This polyclonal antibody was purified using affinity chromatography.
Background:
The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas. BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence.2 Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells,3 suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
BMI-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B501)
Blocking Peptide
BMI-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B501)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human, mouse and rat BMI-1 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
References
1. Alkema, M. J. et al. (1993) Hum. Mol. Genet. 2:1597
2. Jacobs, J. J. et al. (1999) Nature 397:164
3. Lessard, J. et al. (2003) Nature 255
Related Products
BMI-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B501)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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