产品编号:B358
产品描述:Anti-Human Bnip3L
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human Bnip3L
Purified No Carrier Protein
(Nix)
Polyclonal Antibody
Prod. No.: B358
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Bnip3L recognizes an epitope in the intermediate domain of Human Bnip3L. This polyclonal antibody was purified using affinity chromatography.
Background:
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3α, and Nix (for Nip3-like protein X).1-3 Bnip3L/Bnip3α/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis.2,3 Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues.1,2 Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Bnip3L (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B371)
Blocking Peptide
Bnip3L (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B371)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect human and mouse Bnip3L by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
References
1. Matsushima M, Fujiwara T, Takahashi E, et al. Isolation, mapping, and functional analysis of a novel human cDNA (BNIP3L) encoding a protein homologous to human NIP3. Genes Chromosomes Cancer 1998;21:230-5
2. Yasuda M, Han JW, Dionne CA, Boyd JM, Chinnadurai G. BNIP3α: a human homolog of mitochondrial proapoptotic protein BNIP3. Cancer Res 1999;59:533-7
3. Chen G, Cizeau J, Vande Velde C, et al. Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins. J Biol Chem 1999;274(1):7-10
4. Imazu T, Shimizu S, Tagami S, et al. Bcl-2/E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl-2/Bcl-xL and induces apoptosis by altering mitochondrial membrane permeability. Oncogene. 1999;18:4523-9 (RD1200)
Related Products
Bnip3L (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: B371)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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