产品编号:B518

产品描述:Anti-Human BTK(NT)

反应种属:

实验方法:

标记:

规格:0.5mg

供应商:Leinco

价格(RMB):16095.00

订购:

说明书:

Rabbit Anti-Human BTK
Purified No Carrier Protein
(Bruton’s Tyrosine Kinase, Agammaglobulinaemia Tyrosine Kinase, ATK, BPK)
Polyclonal Antibody

Prod. No.: B518
Host:         Rabbit
Conc.:      0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage:     2-8°C

Description
Specificity:
Rabbit Anti-Human BTK recognizes an epitope near the N-terminus of Human and Mouse BTK. This polyclonal antibody was purified using affinity chromatography.

Background:
Bruton’s tyrosine kinase (BTK) was initially identified as a member of the src family for protein-tyrosine kinases that was involved in X-linked aγ-globulinaemia (1), and has since been shown to involved in a number of signaling pathways in hemapoietic lineage (reviewed in 2). It has recently been shown to interact with members of the toll-like receptor (TLR) family such as TLR4, 6, 8, and 9 (3). The TLRs are critical molecules in both the innate and adaptive immunity and can recognize diverse microbial pathogens (reviewed in 4). BTK has also been shown to interact with key proteins involved in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, suggesting that BTK is involved in lipopolysaccharide signal transduction (3).

Known Species Cross Reactivity: Ms+, Hu+
Immunogen
BTK (NT) Blocking peptide (Leinco Prod. No.: B513)
Blocking Peptide
BTK (NT) Blocking peptide (Leinco Prod. No.: B513)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), WB
Western Blotting: This antibody can be used to detect Human and Mouse BTK by Western blot analysis at a concentration of µg/ml. When used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), the detection limit for BTK is 0.5 - 1, under either reducing or non-reducing conditions. The positive control for Western blotting is 3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183).

References
1. Vetrie, D. et al. (1993) Nature 361:226
2. Kawakami, Y. et al. (1999) Biol. 65(3):286
3. Jefferies, C.A. et al. (2003) Biol. Chem. 278:26258
4. Akira, S. et al. (2004) Nat. Rev. Immunol. 4:499
Related Products
BTK (NT) Blocking peptide (Leinco Prod. No.: B513)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.