产品编号:C1224
产品描述:Anti-Human Cbl
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Casitas B-lineage Lymphoma Proto-Oncogene (Cbl)
Purified No Carrier Protein
(Cas-BR-M Mouse Ecotropic Retro-Viral Transforming Sequence )
Polyclonal Antibody
Prod. No.: C1224
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Casitas B-lineage Lymphoma Proto-Oncogene (Cbl) recognizes Human, Mouse and Rat Cbl. This polyclonal antibody was purified using affinity chromatography.
Background:
The mammalian cbl family of ubiquitin ligases consists of three homologs known as cbl (also known as c-Cbl), Cbl-B, and Cbl-3 which share highly conserved a tyrosine-kinase-binding domain, linker and RING finger domain in their amino-terminal halves(reviewed in 1). Similar to other E3 ubiquitin ligases, Cbl catalyzes the transfer of ubiquitin from an E2 or Ubc (ubiquitin-conjugating) enzyme to the e-amino group of a lysine residue of the substrate protein(2). Cbl acts to negatively regulate many types of cell-surface receptors, including the Syk protein tyrosine kinase family. Cbl is thought to be involved in T- and B-cell signaling, in addition to thymus development(3). Of the three known homologs in the cbl family, cbl antibody reacts specifically with cbl. Multiple isoforms of cbl have been reported.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Casitas B-lineage Lymphoma Proto-Oncogene Blocking Peptide (Leinco Prod. No.: C1257)
Blocking Peptide
Casitas B-lineage Lymphoma Proto-Oncogene Blocking Peptide (Leinco Prod. No.: C1257)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat Cbl by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Daudi Whole Cell Lysate (Leinco Prod. No.: D310).
References
1. Thien, C. B. F. et al. (2005) Biochem. J. 391:153
2. Weissman, A. M. (2001) Nat. Rev. Mol. Cell Biol. 2:169
3. Swaminathan, G. et al. (2006) J. Cell. Physiol. 209:21
Related Products
Casitas B-lineage Lymphoma Proto-Oncogene Blocking Peptide (Leinco Prod. No.: C1257)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Daudi Whole Cell Lysate (Leinco Prod. No.: D310)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
