产品编号:C1250
产品描述:Anti-Human cIAP(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human cIAP
Purified No Carrier Protein
(Inhibitor of Apoptosis Protein, HIAP)
Polyclonal Antibody
Prod. No.: C1250
Host: Rabbit
Conc.: 1.0 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human cIAP recognizes an epitope near the C-terminus of mouse and human c-IAP. The antibody detects both c-IAP1 and c-IAP2. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases.1 The two isoforms of c-IAP (c-IAP1 and c-IAP2) are structurally related to XIAP, containing 3 baculoviral IAP repeat (BIR) motifs that are essential and sufficient for the binding and inhibition of caspases–3, –7.2,3 The c-IAPs can associate with the death receptor TNF-R2, and mediate the ubiquitinization of TRAF2 following the binding of TNF-α by its receptor.2,4 Omi, a negative regulator of c-IAP, inhibits its activity by catalytically cleaving c-IAP.5 Another negative regulator, Smac/DIABLO, acts by enhancing the auto-ubiquitization activity of c-IAP.6
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
cIAP (CT) Blocking Peptide (Leinco Prod. No.: C1288)
Blocking Peptide
cIAP (CT) Blocking Peptide (Leinco Prod. No.: C1288)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: To detect Human cIAP this polyclonal antibody can be used at a concentration of 1-2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Human Lung Tissue Lysate (Leinco Prod. No.: H1406).
References
1. Schimmer AD. Inhibitor of apoptosis proteins: translating basic knowledge into clinical practice. Cancer Res. 2004; 64:7183-90.
2. Rothe M, Pan M-G, Henzel WJ, et al. The TNFR2-TRAF signaling complex contains two novel proteins related to baculoviral inhibitor of apoptosis proteins. Cell 1995; 83:1243-52.
3. Deveraux QL, Leo E, Stennicke HR, et al. IAPs block apoptotic events induced by caspase-8 and cytochrome c by direct inhibition of distinct caspases. EMBO J. 1998; 17:2215-23.
4. Li X, Yang Y, Ashwell JD. TNF-RII and c-IAP1 mediate ubiquitinization and degradation of TRAF 2. Nature 2002; 416:345-7.
Related Products
cIAP (CT) Blocking Peptide (Leinco Prod. No.: C1288)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Human Lung Tissue Lysate (Leinco Prod. No.: H1406)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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