产品编号:I-304
产品描述:Anti-Human IKKγ(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human I-Kappa-B Kinase Gamma (IKKγ)
Purified No Carrier Protein
(NEMO, FIP3, IKKAP1)
Polyclonal Antibody
Prod. No.: I-304
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human I-Kappa-B Kinase Gamma (IKKγ) recognizes an epitope near the N-terminus of Human, Mouse and Rat IKKγ. This polyclonal antibody was purified using affinity chromatography.
Background:
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-κB activity. The IkB kinase (IKKα and IKKβ) phosphorylates IkB and mediates NF-κB activation. A novel molecule in the IKK complex was recently identified and termed IKKγ/NEMO/FIP3/IKKAP1.1-4 IKKg interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation.1-4 FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation.3
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
IKKγ (NT) Blocking Peptide (Leinco Prod. No.: I-318)
Blocking Peptide
IKKγ (NT) Blocking Peptide (Leinco Prod. No.: I-318)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat IKKγ by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).
References
1. Rothwarf, D. M. et al. (1998) Nature 395(6699):297
2. Yamaoka, S. et al. (1998) Cell 93(7):1231
3. Li, Y. et al. (1999) Proc. Natl. Acad. Sci. USA 96(3):1042
4. Mercurio, F. et al. (1999) Mol. Cell Biol. 19(2):1526
Related Products
IKKγ (NT) Blocking Peptide (Leinco Prod. No.: I-318)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
I-Kappa-B Kinase Gamma (NT) (IKKγ) (Leinco Prod. No.: I-473)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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