产品编号:I-302
产品描述:Anti-Human IKKβ(CT-3)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human I-Kappa-B-Kinase Beta (IKKβ)
Purified No Carrier Protein
(IKK-2,I-Kappa-B-Kinase 2)
Polyclonal Antibody
Prod. No.: I-302
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human I-Kappa-B-Kinase Beta (IKKβ) recognizes an epitope near the C-terminus of Human IKKβ. This polyclonal antibody was purified using affinity chromatography.
Background:
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories.1-4 IKK is a serine protein kinase, and the IKK complex contains α and β subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for NF-κB activation. IKKβ phosphorylates both IκB-α and IκB-β. IKKβ is expressed in variety of human tissues.
Known Species Cross Reactivity: Hu+
Immunogen
IKKβ (CT-3) Blocking Peptide (Leinco Prod. No.: I-316)
Blocking Peptide
IKKβ (CT-3) Blocking Peptide (Leinco Prod. No.: I-316)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human IKKβ by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are Jurkat Whole Cell Lysate (Leinco Prod. No.: J103) and HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).
References
1. DiDonato, J. A. et al. (1997) Nature 388:548
2. Regnier, C. H. et al. (1997) Cell 90:373
3. Zandi, E,. et al. (1997) Cell 91:243
4. Woronicz, J. D. et al. (1997) Science 278:866
Related Products
IKKβ (CT-3) Blocking Peptide (Leinco Prod. No.: I-316)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Jurkat Whole Cell Lysate (Leinco Prod. No.: J103)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
I-Kappa-B-Kinase Beta (CT-3) Blocking Peptide (Leinco Prod. No.: I-474)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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