产品编号:I-460
产品描述:Anti-Human IKAP
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human IKK Complex-Associated Protein (IKAP)
Purified No Carrier Protein
(IKBKAP)
Polyclonal Antibody
Prod. No.: I-460
Host: Rabbit
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human IKK Complex-Associated Protein (IKAP) recognizes Human and Mouse IKAP. This polyclonal antibody was purified using affinity chromatography.
Background:
IKAP was initially identified as a scaffold protein of the IκB kinase complex that could bind to IKKα, IKKβ, NF-κB, and the NF-κB-inducing kinase (NIK), although later evidence has cast doubt on this. More recent reports show that mutations in IKAP such as a frameshift leading to a truncated protein or a missense mutation that leads to defective phosphorylation are responsible for the autosomal recessive genetic disease familial dysautonomia (FD). Reports indicating that it forms part of the RNA polymerase II transcription elongation complex suggest that this disease may be due to compromised transcription elongation. More recently, it was shown that IKAP associates with c-Jun N-terminal kinase (JNK) and could specifically enhance JNK activation induced by the upstream JNK activators MEKK1 and ASK1, indicating another possible cause for FD. At least two isoforms of IKAP are known two exist.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
IKK Complex-Associated Protein Blocking Peptide (Leinco Prod. No.: I-475)
Blocking Peptide
IKK Complex-Associated Protein Blocking Peptide (Leinco Prod. No.: I-475)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse IKAP by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is A-20 Whole Cell Lysate (Leinco Prod. No.: A232).
References
1. Cohen, L. et al. (1998) Nature 395:292
2. Krappmann, D. et al. (2000) J. Biol. Chem. 275:29779
3. Anderson, S. L. et al. (2001) Am. J. Hum. Genet. ,b>68:753
4. Hawkes, N. A. et al. (2002) J. Biol. Chem. 277:3047
Related Products
IKK Complex-Associated Protein Blocking Peptide (Leinco Prod. No.: I-475)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A-20 Whole Cell Lysate (Leinco Prod. No.: A232)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
.jpg)
