产品编号:I-310

产品描述:Anti-Human IRAK-M

反应种属:

实验方法:

标记:

规格:0.5mg

供应商:Leinco

价格(RMB):16095.00

订购:

说明书:

Rabbit Anti-Human IL-1 Receptor Associated Kinase M (IRAK-M)
Purified No Carrier Protein
Polyclonal Antibody

Prod. No.: I-310
Host:         Rabbit
Conc.:      0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human IL-1 Receptor Associated Kinase M (IRAK-M) recognizes Human, Mouse and Rat IRAK-M. This polyclonal antibody was purified using affinity chromatography.

Background:
Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-βB by IL-1/Toll receptors,1,2 which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M.3 IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-βB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
IRAK-M Blocking Peptide (Leinco Prod. No.: I-322)
Blocking Peptide
IRAK-M Blocking Peptide (Leinco Prod. No.: I-322)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat IRAK-M by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are Mouse Spleen Tissue Lysate (Leinco Prod. No.: M1018) and Rat Kidney Tissue Lysate (Leinco Prod. No.: R1013).

References
1. Cao, Z. et al. (1996) Science 271:1128
2. Muzio, M. et al. (1997) Science 278:1612
3. Wesche, H. <et al.> (1999) J. Biol. Chem. 274(27):19403
Related Products
IRAK-M Blocking Peptide (Leinco Prod. No.: I-322)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Mouse Spleen Tissue Lysate (Leinco Prod. No.: M1018)
Rat Kidney Tissue Lysate (Leinco Prod. No.: R1013)
PN:

Products are for research use only. Not for use in diagnostic or therapeutic procedures.