产品编号:I-307
产品描述:Anti-Human IRAK(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human IL-1 Receptor-Associated Kinase (IRAK)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: I-307
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human IL-1 Receptor-Associated Kinase (IRAK) recognizes Human, Mouse and Rat IRAK. This polyclonal antibody was purified using affinity chromatography.
Background:
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
IRAK (CT) Blocking Peptide (Leinco Prod. No.: I-321)
Blocking Peptide
IRAK (CT) Blocking Peptide (Leinco Prod. No.: I-321)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), IP, WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat IRAK by Western blot analysis at a dilution of 1:1000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are HeLa Whole Cell Lysate (Leinco Prod. No.: H1402) and THP-1 Whole Cell Lysate (Leinco Prod. No.: T206).
References
1. Cao, Z. et al. (1996) Science 271:1128
2. Trofimova, M. et al. (1996) J. Bio. Chem. 271:17609
3. Jianing, Huang et al. (1997) Proc. Natl. Acad. Sci. USA 94:12829
4. Robinson, D. et al. (1997) Immunity 7:571
Related Products
IRAK (CT) Blocking Peptide (Leinco Prod. No.: I-321)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
THP-1 Whole Cell Lysate (Leinco Prod. No.: T206)
PN:
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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