产品编号:I-458
产品描述:Anti-Human 4E-BP1
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Initiation Factor 4E Binding Protein 1 (4E-BP1)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: I-458
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Initiation Factor 4E Binding Protein 1 (4E-BP1) recognizes Human and Mouse 4E-BP1. This polyclonal antibody was purified using affinity chromatography.
Background:
The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms (reviewed in 1). One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 2). One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5’-UTRs.1 Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 3), at least in part by inhibiting the activity of TOR towards 4E-BP1.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Initiation Factor 4E Binding Protein 1 Blocking Peptide (Leinco Prod. No.: I-459)
Blocking Peptide
Initiation Factor 4E Binding Protein 1 Blocking Peptide (Leinco Prod. No.: I-459)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse 4E-BP1 by Western blot analysis at a concentration of 2.5-5.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is 3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183).
References
1. Shah, O. J. et al. (2000) Am. J. Physiol. Endocrinol. Metab. 279:E715
2. Shamji, A. F. et al. (2003) Mol. Cell 12:271
Related Products
Initiation Factor 4E Binding Protein 1 Blocking Peptide (Leinco Prod. No.: I-459)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
