产品编号:I-465
产品描述:Anti-Human IRS-1
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Insulin Receptor Substrate-1 (IRS-1)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: I-465
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Insulin Receptor Substrate-1 (IRS-1) recognizes Human and Mouse IRS-1. This polyclonal antibody was purified using affinity chromatography.
Background:
Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins.1 IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-α, in addition to the insulin and insulin-like growth factor 1 receptors.2 The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR)>3,4 TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway.3
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Insulin Receptor Substrate-1 Blocking Peptide (Leinco Prod. No.: I-482)
Blocking Peptide
Insulin Receptor Substrate-1 Blocking Peptide (Leinco Prod. No.: I-482)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse IRS-1 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is PC-3 Whole Cell Lysate (Leinco Prod. No.: P166).
References
1. Giovannone, B. et al. (2000) Diabetes Metab. Res. Rev. 16:434
2. Waters, S. B. et al. (1996) Trends in Cell Biol. 6:1
3. Ozes, O. N. et al. (2001) Proc. Natl. Acad. Sci. USA 98:4640
4. Shamji, A. F. et al. (2003) Mol. Cell 12:271
Related Products
Insulin Receptor Substrate-1 Blocking Peptide (Leinco Prod. No.: I-482)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
PC-3 Whole Cell Lysate (Leinco Prod. No.: P166)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
