产品编号:I-490
产品描述:Anti-Human Ipaf
反应种属:
实验方法:
标记:
规格:0.1mg
供应商:Leinco
价格(RMB):5370.00
订购:
说明书:
Rabbit Anti-Human Ipaf
Purified No Carrier Protein
(ICE Protease Activating Factor, CARD12, CLANA )
Polyclonal Antibody
Prod. No.: I-490
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Ipaf recognizes an epitope near the C-terminus of human Ipaf. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NF-κB signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family.1 Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner.2,3 Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.
Known Species Cross Reactivity: Hu+
Immunogen
Ipaf Blocking Peptide (Leinco Prod. No.: I-497)
Blocking Peptide
Ipaf Blocking Peptide (Leinco Prod. No.: I-497)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), IHC (Frozen), WB
Western Blotting: This antibody can be used to detect Human Ipaf by Western blot analysis at a concentration of 0.5-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Peripheral Blood Leukocyte (PBL) Tissue Lysate (Leinco Prod. No.: P180).
References
1. Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.
2. Poyet J-L, Srinivaula SM, Tnani M, et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. J. Biol. Chem. 2001; 276:28309-13.
3. Geddes BJ, Wang L, Huang WJ, et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochem. Biophys. Res. Commun. 2001; 284:77-82.
Related Products
Ipaf Blocking Peptide (Leinco Prod. No.: I-497)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Peripheral Blood Leukocyte (PBL) Tissue Lysate (Leinco Prod. No.: P180)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
