产品编号:I-461

产品描述:Anti-Human IRAK-4

反应种属:

实验方法:

标记:

规格:0.5mg

供应商:Leinco

价格(RMB):16095.00

订购:

说明书:

Rabbit Anti-Human IRAK-4
Purified No Carrier Protein
Polyclonal Antibody

Prod. No.: I-461
Host:        Rabbit
Conc.:      0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C 

Description
Specificity:
Rabbit Anti-Human IRAK-4 recognizes an epitope near the C-terminus of Human IRAK-4. This polyclonal antibody was purified using affinity chromatography.

Background:
Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular responses through IL-1 receptor (IL-1R) and Toll-like receptors (TLR). IL-1R-associated kinases (IRAK, IRAK2, and IRAK-M) regulate the activation of NF-κB and MAP kinase (MAPK) by IL-1R/TLR (1-3). A novel member in the IRAK/Pelle family was recently identified and designated IRAK-4.4 Overexpression of IRAK-4 activates NF-kB and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.

Known Species Cross Reactivity: Hu+
Immunogen
IRAK-4 Blocking Peptide (Leinco Prod. No.: I-483)
Blocking Peptide
IRAK-4 Blocking Peptide (Leinco Prod. No.: I-483)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human IRAK-4 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).

References
1. Cao, Z. et al. (1996) Science 271:1128
2. Muzio, M. et al. (1997) Science 278:1612
3. Wesche, H. <et al.> (1999) J. Biol. Chem. 274(27):19403
4. Li, S. et al. (2002) Proc. Natl. Acad. Sci. USA 99(8):5567
Related Products
IRAK-4 Blocking Peptide (Leinco Prod. No.: I-483)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.