产品编号:I-463
产品描述:Anti-Human IRE1p(Intermediate Domain)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human IRE1p
Purified No Carrier Protein
(Endoplasmic Reticulum-to-Nucleus Signaling 1, ERN1 )
Polyclonal Antibody
Prod. No.: I-463
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human IRE1p recognizes an epitope in the intermediate domain of Human IRE1p. This polyclonal antibody was purified using affinity chromatography.
Background:
Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94.1,2 These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF63,4 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation.5 Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.6 Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Known Species Cross Reactivity: Hu+
Immunogen
IRE1p (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: I-485)
Blocking Peptide
IRE1p (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: I-485)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human IRE1p by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Raji Whole Cell Lysate (Leinco Prod. No.: R1009).
References
1. Takeda, K. et al. (2003) Annu. Rev. Immunol. 21:335
2. Lee, A. S. (2005) Methods 35:373
3. Bertolotti, A. et al. (2000) Nat. Cell Biol. 2:326
4. Shen, J. et al. (2002) Dev. Cell 3:99
5. Lee, K. et al. (2002) Genes Dev. 16:452
6. Miyoshi, K. et al. (2000) Res. Mol. Brain Res. 85:68
Related Products
IRE1p (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: I-485)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Raji Whole Cell Lysate (Leinco Prod. No.: R1009)
Anti-Human IRE1p (CT) (Leinco Prod. No.: I-462)
Anti-Human IRE1p (NT) (Leinco Prod. No.: I-464)
IRE1p (CT) Blocking Peptide (Leinco Prod. No.: I-484)
IRE1p (NT) Blocking Peptide (Leinco Prod. No.: I-486)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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