产品编号:I-466
产品描述:Anti-Human Lipe
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Lipe
Purified No Carrier Protein
(Hormone-Sensitive Lipase, HSL )
Polyclonal Antibody
Prod. No.: I-466
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Lipe recognizes Human, Mouse and Rat Lipe. This polyclonal antibody was purified using affinity chromatography.
Background:
Although initially described as an adipocyte-specific triacylglycerol lipase, lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that lipe plays a major role in adipocyte proliferation.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Lipe Blocking Peptide (Leinco Prod. No.: I-487)
Blocking Peptide
Lipe Blocking Peptide (Leinco Prod. No.: I-487)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat Lipe by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Lymph Node Normal Tissue Lysate (Leinco Prod. No.: L161).
References
1. Yeaman, S. J. (2004) Biochem. J. 379:11
2. Holst, L. S. et al. (1996) Genomics 35:441
3. Kraemer, F. B. et al. (2004) Mol. Endocrinol. 18:549
4. Sekiya, M. et al. (2004) J. Biol. Chem. 279:15084
Related Products
Lipe Blocking Peptide (Leinco Prod. No.: I-487)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Lymph Node Normal Tissue Lysate (Leinco Prod. No.: L161)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
