产品编号:L169
产品描述:Anti-Human LAMP-2
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Lysosome Associated Membrane Protein 2 (LAMP-2)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: L169
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Lysosome Associated Membrane Protein 2 (LAMP-2) recognizes mouse and human LAMP-2. This polyclonal antibody was purified using affinity chromatography.
Background:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components1,2 and is negatively regulated by TOR (Target of rapamycin).3 LAMP-2, a highly glycosylated protein associated with the lysosome,4 has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon’s disease is associated with the accumulation of autophagic material in striated myocytes. LAMP-2 exists in multiple isoforms.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Lysosome Associated Membrane Protein 2 Blocking Peptide (Leinco Prod. No.: L171)
Blocking Peptide
Lysosome Associated Membrane Protein 2 Blocking Peptide (Leinco Prod. No.: L171)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), IHC (Frozen), WB
Western Blotting: This antibody can be used to detect mouse and human LAMP-2 by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HepG2 Whole Cell Lysate (Leinco Prod. No.: H1404).
References
1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4. Granger BL, Green SA, Gabel CA, et al. Characterization and cloning of the lgp110, a lysosomal glycoprotein from mouse and rat cells. J. Biol. Chem. 1990; 265:12036-43. 5. Tanaka Y, Guhde G, Suter A, et al. Accumulation of autophagic vacuoles and cardiopathy in LAMP-2-deficient mice. Nature 2000; 902-6. 6. Nishino I, Fu J, Tanji K, et al. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature 2000; 906-10. 7. Gough NR, Hatem CL, and Fambrough DM. The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c. DNA Cell Biol. 1995; 14:863-7.
Related Products
Lysosome Associated Membrane Protein 2 Blocking Peptide (Leinco Prod. No.: L171)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HepG2 Whole Cell Lysate (Leinco Prod. No.: H1404)
Anti-Human Lysosome Associated Membrane Protein 1 (LAMP-1) (Leinco Prod. No.: L168)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
