产品编号:M1021

产品描述:Anti-Human MADD(CT)

反应种属:

实验方法:

标记:

规格:0.5mg

供应商:Leinco

价格(RMB):16095.00

订购:

说明书:

Rabbit Anti-Human MAP Kinase-Activating Death Domain (MADD)
Purified No Carrier Protein
(DENN)

Prod. No.: M1021
Host:         Rabbit
Conc.:      0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human MAP Kinase-Activating Death Domain (MADD) recognizes Human, Mouse and Rat MADD. This polyclonal antibody was purified using affinity chromatography.

Background:
MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98% identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94% overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87% identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
MAP Kinase-Activating Death Domain (CT) Blocking Peptide (Leinco Prod. No.: M1207)
Blocking Peptide
MAP Kinase-Activating Death Domain (CT) Blocking Peptide (Leinco Prod. No.: M1207)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human MADD by Western blot analysis at a dilution of 1:250-1:500 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are HeLa Whole Cell Lysate (Leinco Prod. No.: H1402) and 3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183).

References
1. Schievella, A. R. et al. (1997) J. Biol. Chem. 272:12069
2. Chow, V. T. et al. (1996) DNA Seq. 6:263
3. Zhang, Y. et al. (1998) Proc. Natl. Acad. Sci. USA 95:2586
4. Brown, T. L. et al. (1998) Curr. Biol. 8:R191
Related Products
MAP Kinase-Activating Death Domain (CT) Blocking Peptide (Leinco Prod. No.: M1207)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183)
PN:

Products are for research use only. Not for use in diagnostic or therapeutic procedures.