产品编号:M1212
产品描述:Anti-Human Mcl-1(Intermediate Domain)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Mcl-1
Purified No Carrier Protein
(Myeloid Cell Leukemia-1)
Polyclonal Antibody
Prod. No.: M1212
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Mcl-1 recognizes an epitope in the intermediate domain of Human Mcl-1. This polyclonal antibody was purified using affinity chromatography.
Background:
Myeloid cell leukimia-1 (Mcl-1) is a member of the Bcl-2 family of proteins that can act to promote cell survival (reviewed in 1). While the mechanism by which Mcl-1 inhibits apoptosis is not known, it is thought that it may heterodimerize and neutralize pro-apoptotic members of the Bcl-2 family such as Bim or Bak.2,3 Mcl-1 was originally identified in differentiating myeloid cells, but has since been shown to be expressed in multiple cell types. Mcl-1 is essential for embryogenesis and for the development and maintenance of B and T lymphocytes in animals.3 Mcl-1 exists as at least two distinct isoforms designated Mcl-1L and Mcl-1S. In marked contrast to the larger isoform of Mcl-1, overexpression of Mcl-1S promotes cell death.
Known Species Cross Reactivity: Hu+
Immunogen
Mcl-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1216)
Blocking Peptide
Mcl-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1216)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human Mcl-1 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Raji Whole Cell Lysate (Leinco Prod. No.: R1009).
References
1. Edwards, S. W. et al. (2004) Biochem Soc Trans. 32:489
2. Cuconati, A. et al. (2003) Genes and Dev. 17:2922
3. Opferman, J. T. et al. (2003) Nature 426:671
4. Kozopas, K. M. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3516
Related Products
Mcl-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1216)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Raji Whole Cell Lysate (Leinco Prod. No.: R1009)
Anti-Human Mcl-1 (CT) (Leinco Prod. No.: M1211)
Anti-Human Mcl-1 (NT) (Leinco Prod. No.: M1213)
Mcl-1 (CT) Blocking Peptide (Leinco Prod. No.: M1215)
Mcl-1 (NT) Blocking Peptide (Leinco Prod. No.: M1217)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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