产品编号:M1219
产品描述:Anti-Human MD-1(Intermediate Domain)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human MD-1
Purified No Carrier Protein
(Lymphocyte Antigen 86 )
Polyclonal Antibody
Prod. No.: M1219
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human MD-1 recognizes an epitope near the center of human, mouse and rat MD-1. This polyclonal antibody was purified using affinity chromatography.
Background:
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1). The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins such as RP105, a recently identified homolog of TLR4. This protein, in association with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway (2). While MD-2 can directly bind to LPS, one of the activating molecules of TLR4, the function of MD-1 is less well-known. It has been suggested however, that the RP105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes (4).
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
MD-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1223)
Blocking Peptide
MD-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1223)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB, In vivo Depletion
Western Blotting: This antibody can be used to detect human, mouse and rat MD-1 by Western blot analysis at a concentration of 1 - 2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Daudi Whole Cell Lysate (Leinco Prod. No.: D310).
References
1. Takeda, K. et al. (2003) Annu. Rev. Immunol. 21:335
2. Divanovic, S. et al. (2005) J. Endotoxin Res. 11:363
3. Tsuneyoshi, N. et al. (2005) J. Immunol. 174/b>:340
4. Nagai, Y. et al. (2005) J. Immunol. 174/b>:7043
Related Products
MD-1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1223)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Daudi Whole Cell Lysate (Leinco Prod. No.: D310)
Anti-Human MD-1 (CT) (Leinco Prod. No.: M1218)
MD-1 (CT) Blocking Peptide (Leinco Prod. No.: M1222)
Anti-Mouse MD-1 (Antigen Aff. Pur.) (Leinco Prod. No.: M1260)
Anti-Mouse MD-1 (Leinco Prod. No.: M1268)
Anti-Mouse MD-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: M1304)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
