产品编号:M1210
产品描述:Anti-Human MAP1
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Modulator of Apoptosis 1 (MAP1)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: M1210
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Modulator of Apoptosis 1 (MAP1) recognizes an epitope in the intermediate domain of Human and Mouse MAP1. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains (reviewed in 2 and 3). A related protein termed MAP-1 has recently been identified. This protein contains a BH3-like domain and induces caspase-dependent apoptosis in mammalian cells when overexpressed. It forms homodimers and associates with Bcl-2 family members such as Bax, Bcl-2, and Bcl-XL in vitro and in vivo.4 It has been suggested that MAP-1 associates with the tumor suppressor RASSF1A following death receptor activation, allowing a conformational change in Bax that leads to cellular apoptosis.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
Modulator of Apoptosis 1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1214)
Blocking Peptide
Modulator of Apoptosis 1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1214)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human and Mouse MAP1 by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is EL4 Whole Cell Lysate (Leinco Prod. No.: E153).
References
1. Lockshin, R. A. et al. (2000) Cell Death Differ. 7:2
2. Cory, S. et al. (2003) Oncogene 22:8590
3. Heiser, D. et al. (2004) Exp. Geron. 39:1125
4. Tan, K. O. et al. (2001) J. Biol. Chem. 276:2802
Related Products
Modulator of Apoptosis 1 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1214)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
EL4 Whole Cell Lysate (Leinco Prod. No.: E153)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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