产品编号:M1023
产品描述:Anti-Human MyD88(Intermediate Domain)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human MyD88
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: M1023
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human MyD88 recognizes an epitiope near the intermediate domain of Human MyD88. This polyclonal antibody was purified using affinity chromatography.
Background:
The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway.1 MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-κB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor,2,3 which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
Known Species Cross Reactivity: Hu+
Immunogen
MyD88 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1209)
Blocking Peptide
MyD88 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1209)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human MyD88 by Western blot analysis at a concentration of 1:500-1:1000 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are Jurkat Whole Cell Lysate (Leinco Prod. No.: J103) and Human Heart Tissue Lysate (Leinco Prod. No.: H1405).
References
1. Muzio, M. et al. (1997) Science 278:1612
2. Adachi, O. et al. (1998) Immunity 9:143
3. Medzhitov, R. et al. (1998) Mol. Cell 2:253
Related Products
MyD88 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1209)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Jurkat Whole Cell Lysate (Leinco Prod. No.: J103)
Human Heart Tissue Lysate (Leinco Prod. No.: H1405)
MyD88 (CT) Blocking Peptide (Leinco Prod. No.: M1010)
MyD88 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: M1011)
Anti-Human MyD88 (CT) (Leinco Prod. No.: M1022)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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