产品编号:N155
产品描述:Anti-Human NADE
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human NADE
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: N155
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human NADE recognizes Human and Mouse NADE. This polyclonal antibody was purified using affinity chromatography.
Background:
The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF) (1 for review). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR.2 Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells,3 NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.4
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
NADE Blocking Peptide (Leinco Prod. No.: N168)
Blocking Peptide
NADE Blocking Peptide (Leinco Prod. No.: N168)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human and Mouse NADE by Western blot analysis at a concentration of 0.5-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Brain Tissue Lysate (Leinco Prod. No.: H1407).
References
1. Gentry, J. J. et al. (2004) Pro. Brain Res. 146:25
2. Mukai, J. et al. (2000) J. Biol. Chem. 275:17566
3. Rapp, G. et al. (1990) DNA Cell Biol. 9:479
4. Yi, J. S. et al. (2003) Neurosci. Lett. 347:126
Related Products
NADE Blocking Peptide (Leinco Prod. No.: N168)
Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114)
Human Brain Tissue Lysate (Leinco Prod. No.: H1407)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.