产品编号:N159
产品描述:Anti-Human NOD2(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human NOD2
Purified No Carrier Protein
(Caspase Recruitment Domain 15, CARD15 )
Polyclonal Antibody
Prod. No.: N159
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human NOD2 recognizes an epitope near the C-terminus of Human NOD2. This polyclonal antibody was purified using affinity chromatography.
Background:
Apaf-1 and NOD1 are members of a new family,1 which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2.2 NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn’s disease3,4 that is a main type of chronic inflammatory bowel disease.
Known Species Cross Reactivity: Hu+
Immunogen
NOD2 (CT) Blocking Peptide (Leinco Prod. No.: N172)
Blocking Peptide
NOD2 (CT) Blocking Peptide (Leinco Prod. No.: N172)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human NOD2 by Western blot analysis at a concentration of 2.0-4.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is HeLa Whole Cell Lysate (Leinco Prod. No.: H1402).
References
1. Inohara, N. et al. (1999) J. Biol. Chem. 274:14560
2. Ogura, Y. et al. (2001) J. Biol. Chem. 276:4812
3. Hugot, J. P. et al. (2001) Nature 411:599
4. Ogura, Y. et al. (2001) Nature 411:603
Related Products
NOD2 (CT) Blocking Peptide (Leinco Prod. No.: N172)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
HeLa Whole Cell Lysate (Leinco Prod. No.: H1402)
Anti-Human NOD2 (NT) (Leinco Prod. No.: N160)
NOD2 (NT) Blocking Peptide (Leinco Prod. No.: N173)
Products are for research use only. Not for use in diagnostic or therapeutic procedures
.jpg)
.jpg)
