产品编号:N161
产品描述:Anti-Human Noxa
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Noxa
Purified No Carrier Protein
(PMA-Induced Protein 1, PMAIP1, APR)
Polyclonal Antibody
Prod. No.: N161
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Noxa recognizes Human, Mouse and Rat Noxa. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53.1-3 Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.4
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Noxa Blocking Peptide (Leinco Prod. No.: N174)
Blocking Peptide
Noxa Blocking Peptide (Leinco Prod. No.: N174)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat Noxa by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Stomach Tissue Lysate (Leinco Prod. No.: S516).
References
1. Oda, E. et al. (2000) Science 288:1053
2. Nakano, K. et al. (2001) Mol. Cell 7:683
3. Yu, J. et al. (2001) Mol. Cell 7:673
4. Eferl, R. et al. (2003) Cell 112:181
Related Products
Noxa Blocking Peptide (Leinco Prod. No.: N174)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Stomach Tissue Lysate (Leinco Prod. No.: S516)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
