产品编号:O113
产品描述:Anti-Human OMI(CT-2)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human OMI
Purified No Carrier Protein
(HtrA2)
Polyclonal Antibody
Prod. No.: O113
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human OMI recognizes an eptiope near the C-terminus of Human OMI. This polyclonal antibody was purified using affinity chromatography.
Background:
Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication.1 Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases (reviewed in 2). The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs (reviewed in 3). Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs.4 Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis.
Known Species Cross Reactivity: Hu+
Immunogen
OMI (CT-2) Blocking Peptide (Leinco Prod. No.: O116)
Blocking Peptide
OMI (CT-2) Blocking Peptide (Leinco Prod. No.: O116)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), IHC (Frozen), WB
Western Blotting: This antibody can be used to detect Human OMI by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is U937 Whole Cell Lysate (Leinco Prod. No.: U123).
References
1. Crook, N. E. et al. (1993) J. Virol. 67:2168
2. Liston, P. et al. (2003) Oncogene 22:8568
3. Vaux, D. L. et al. (2003) Biochem. Biophys. Res. Comm. 304:499
4. Suzuki, Y. et al. (2001) Mol. Cell 8:613
Related Products
OMI (CT-2) Blocking Peptide (Leinco Prod. No.: O116)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
U937 Whole Cell Lysate (Leinco Prod. No.: U123)
Anti-Human OMI (CT-1) (Leinco Prod. No.: O114)
OMI (CT-1) Blocking Peptide (Leinco Prod. No.: O115)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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