产品编号:P192
产品描述:Anti-Human PAK2(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human P21-Activated Kinase 2 (PAK2)
Purified No Carrier Protein
(PAK65)
Polyclonal Antibody
Prod. No.: P192
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human P21-Activated Kinase 2 (PAK2) recognizes an epitope near the N-terminus of Human, Mouse and Rat PAK2. This polyclonal antibody was purified using affinity chromatography.
Background:
The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
P21-Activated Kinase 2 (NT) Blocking Peptide (Leinco Prod. No.: P199)
Blocking Peptide
P21-Activated Kinase 2 (NT) Blocking Peptide (Leinco Prod. No.: P199)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat PAK2 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Jurkat Whole Cell Lysate (Leinco Prod. No.: J103).
References
1. Jaffer, Z. M. et al. (2004) Cell Biol. 34:713
2. Rudel, T. et al. (1997) Science 276:1571
3. Vilas, G. L. et al. (2006) Proc. Natl. Acad. Sci. USA 103:6542
Related Products
P21-Activated Kinase 2 (NT) Blocking Peptide (Leinco Prod. No.: P199)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Jurkat Whole Cell Lysate (Leinco Prod. No.: J103)
Anti-Human P21-Activated Kinase 2 (CT) (PAK2) (Leinco Prod. No.: P191)
P21-Activated Kinase 2 (CT) Blocking Peptide (Leinco Prod. No.: P198)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
.jpg)
