产品编号:P194
产品描述:Anti-Human PAK5
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human P21-Activated Kinase 5 (PAK5)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: P194
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human P21-Activated Kinase 5 (PAK5) recognizes Human PAK5. This polyclonal antibody was purified using affinity chromatography.
Background:
The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK5 was initially identified as a kinase expressed primarily in brain that while possessing a kinase domain and GTPase binding domain similar to PAK4 and PAK6, is completely different from both. Expression of PAK5 in neural based cell lines resulted in neurite outgrowth suggesting that PAK5 may be involved in regulating the cytoskeletal changes necessary for promoting neurite outgrowth. Other experiments suggest that unlike the other PAKs, PAK5 may inhibit apoptosis by phosphorylating the Bcl-2 family member Bad.
Known Species Cross Reactivity: Hu+
Immunogen
P21-Activated Kinase 5 Blocking Peptide (Leinco Prod. No.: P201)
Blocking Peptide
P21-Activated Kinase 5 Blocking Peptide (Leinco Prod. No.: P201)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human PAK5 by Western blot analysis at a concentration of 2.0-4.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is T24 Whole Cell Lysate (Leinco Prod. No.: T207).
References
1. Jaffer, Z. M. et al. (2002) Int. J. Biochem. Cell Biol. 34:713
2. Dan, C. et al. (2002) Mol. Cell. Biol. 22:567
3. Cotteret, S. et al. (2003) Mol. Cell. Biol. 23:5526
Related Products
P21-Activated Kinase 5 Blocking Peptide (Leinco Prod. No.: P201)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
T24 Whole Cell Lysate (Leinco Prod. No.: T207)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
