产品编号:P157
产品描述:Anti-Human PUMA(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human p53 Upregulated Modulator of Apoptosis (PUMA)
Purified No Carrier Protein
(bbc3)
Polyclonal Antibody
Prod. No.: P157
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human p53 Upregulated Modulator of Apoptosis (PUMA) recognizes an epitope near the N-terminus of Human PUMA. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse.1-3 PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β1 PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Known Species Cross Reactivity: Hu+
Immunogen
p53 Upregulated Modulator of Apoptosis (NT) Blocking Peptide (Leinco Prod. No.: P158)
Blocking Peptide
p53 Upregulated Modulator of Apoptosis (NT) Blocking Peptide (Leinco Prod. No.: P158)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human PUMA by Western blot analysis at a concentration of 2.0-4.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is K562 Whole Cell Lysate (Leinco Prod. No.: K128).
References
1. Nakano, K. et al. (2001) Mol Cell. 7(3):683
2. Han, J. et al. (2001) Proc. Natl. Acad. Sci. USA 98(20):11318
Related Products
p53 Upregulated Modulator of Apoptosis (NT) Blocking Peptide (Leinco Prod. No.: P158)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
p53 Upregulated Modulator of Apoptosis (CT) Blocking Peptide (Leinco Prod. No.: P156)
Anti-Human p53 Upregulated Modulator of Apoptosis (CT) (PUMA) (Leinco Prod. No.: P155)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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