产品编号:P208
产品描述:Anti-Human PHAP(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human PHAP
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: P208
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human PHAP recognizes an epitope near the N-terminus of Human, Mouse and Rat PHAP. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis.1 PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
PHAP (NT) Blocking Peptide (Leinco Prod. No.: P218)
Blocking Peptide
PHAP (NT) Blocking Peptide (Leinco Prod. No.: P218)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat PHAP by Western blot analysis at a concentration of 0.5-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Raji Whole Cell Lysate (Leinco Prod. No.: R1009).
References
1. Jiang, X. et al. (2003) Science 299(5604):223
2. Nicholson, D. W. et al. (2003) Science 10;299(5604):214
Related Products
PHAP (NT) Blocking Peptide (Leinco Prod. No.: P218)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Raji Whole Cell Lysate (Leinco Prod. No.: R1009)
Anti-Human PHAP (CT) (Leinco Prod. No.: P207)
PHAP (CT) Blocking Peptide (Leinco Prod. No.: P217)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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