产品编号:P211

产品描述:Anti-Human PHAP III(CT)

反应种属:

实验方法:

标记:

规格:0.5mg

供应商:Leinco

价格(RMB):16095.00

订购:

说明书:

Rabbit Anti-Human PHAP III
Purified No Carrier Protein
Polyclonal Antibody

Prod. No.: P211
Host:        Rabbit
Conc.:      0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human PHAP III recognizes an epitope near the C-terminus of Human, Mouse and Rat PHAP III. This polyclonal antibody was purified using affinity chromatography.

Background:
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis.1 PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.

Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
PHAP III Blocking Peptide (Leinco Prod. No.: P221)
Blocking Peptide
PHAP III Blocking Peptide (Leinco Prod. No.: P221)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat PHAP III by Western blot analysis at a concentration of 0.5-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are A549 Whole Cell Lysate (Human Lung Carcinoma) (Leinco Prod. No.: A181) and HepG2 Whole Cell Lysate (Leinco Prod. No.: H1404).

References
1. Jiang, X. et al. (2003) Science 299(5604):223
2. Nicholson, D. W. et al. (2003) Science 10;299(5604):214
Related Products
PHAP III Blocking Peptide (Leinco Prod. No.: P221)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A549 Whole Cell Lysate (Human Lung Carcinoma) (Leinco Prod. No.: A181)
HepG2 Whole Cell Lysate (Leinco Prod. No.: H1404)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.