产品编号:P212
产品描述:Anti-Human PIKE(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human PIKE
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: P212
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human PIKE recognizes an epitope near the C-terminus of Human and Mouse PIKE. This polyclonal antibody was purified using affinity chromatography.
Background:
Phosphoinositide 3 kinase enhancer (PIKE) is a recently identified nuclear GTPase that interacts with nuclear phosphoinositide 3-kinase (PI3 kinase) to stimulate its lipid kinase activity (1). PIKE exists in multiple isoforms; a shorter C-terminal isoform (PIKE-A) has also been identified as centaurin γ 1 (reviewed in 2). The longest isoform (PIKE-L) has been shown to bind to the adaptor protein Homer and thereby link to metabotropic glutamate receptors, leading to activation of PI3 kinase activity and prevention of neuronal apoptosis.3 Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion, whereas decreased expression of PIKE-A via dominant negative expression of PIKE-A or PIKE-A knockdown inhibits these processes.4 In many human cancers, expression of PIKE-A is enhanced, leading to increased Akt activity and preventing apoptosis.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
PIKE (CT) Blocking Peptide (Leinco Prod. No.: P222)
Blocking Peptide
PIKE (CT) Blocking Peptide (Leinco Prod. No.: P222)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), IHC (Frozen), WB
Western Blotting: This antibody can be used to detect Human and Mouse PIKE by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Mouse Brain Tissue Lysate (Leinco Prod. No.: M1015).
References
1. Ye, K. et al. (2000) Cell 103:919
2. Jackson, T. R. et al. (2000) Trends Biochem. Sci. 25:489
3. Rong, R. et al. (2003) Nat. Neurosci. 6:1153
4. Ahn, J. Y. et al. (2004) J. Biol. Chem. 279:16441
Related Products
PIKE (CT) Blocking Peptide (Leinco Prod. No.: P222)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Mouse Brain Tissue Lysate (Leinco Prod. No.: M1015)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
.jpg)
.jpg)
