产品编号:P213
产品描述:Anti-Human PIST(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human PIST
Purified No Carrier Protein
(Golgi Associated PDZ and Coiled-coil Motif Containing/GOPC )
Polyclonal Antibody
Prod. No.: P213
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human PIST recognizes an epitope near the C-terminus of Human, Mouse and Rat PIST. This polyclonal antibody was purified using affinity chromatography.
Background:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components1,2 and is negatively regulated by TOR (Target of rapamycin).3 PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6.4 Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
PIST (CT) Blocking Peptide (Leinco Prod. No.: P223)
Blocking Peptide
PIST (CT) Blocking Peptide (Leinco Prod. No.: P223)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat PIST by Western blot analysis at a concentration of 1.0-2.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Rat Colon Tissue Lysate (Leinco Prod. No.: R1166).
References
1. Gozuacik, D. et al. (2004) Oncogene 23:2891
2. Kisen, G. O. et al. (1993) Carcinogenesis 14:2501
3. Kamada, Y. et al. (2000) J. Cell. Biol. 150:1507
4. Yu, Z. et al. (2002) Neuron 35:921
Related Products
PIST (CT) Blocking Peptide (Leinco Prod. No.: P223)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Rat Colon Tissue Lysate (Leinco Prod. No.: R1166)
Anti-Human PIST (Intermediate Domain) (Leinco Prod. No.: P214)
PIST (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: P224)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.