产品编号:P228
产品描述:Anti-Human PKR(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human PKR
Purified No Carrier Protein
(Protein Kinase RNA-activated, Interferon-inducible RNA-dependent Protein Kinase)
Polyclonal Antibody
Prod. No.: P228
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human PKR recognizes an epitope near the C-terminus of human, mouse and rat PKR. This polyclonal antibody was purified using affinity chromatography.
Background:
The interferon-inducible, double-stranded RNA (dsRNA)- dependent protein kinase PKR is a member of the eukaryotic initiatio n factor-2 alpha (eIF2-α) kinase family, possessing serine-threonine kinase activity and two dsRNA-binding motifs (1) that acts as part of the innate immune system. Upon binding dsRNA, PKR undergoes a conformational change leading to its activation and its phosphorylation of the translation factor eIF2, resulting in a general shutdown of protein synthesis (2) and induction of apoptosis through upregulation of caspase-8 and capsase-9 activity (3,4) in order to prevent the production of more viruses. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the nonstructural protein (NS1) of the influenza virus (5). More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis (6).
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
PKR (CT) Blocking Peptide (Leinco Prod. No.: P230)
Blocking Peptide
PKR (CT) Blocking Peptide (Leinco Prod. No.: P230)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB, In vivo Depletion
Western Blotting: This antibody can be used to detect human, mouse and rat PKR by Western blot analysis at a concentration of 0.5 - 1 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is A431 Whole Cell Lysate (Human Epidermoid Carcinoma) (Leinco Prod. No.: A180).
References
1. Meurs E, Chong K, Galabru J, et al. Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon. Cell 1990; 62 :379-90.
2. Saelens X, Kalai M, and Vandenabeele P. Translation inhibition in apoptosis: Caspase-dependent PKR activation and eIF2-alpha phosphorylation. J. Biol. Chem. 2001; 276:41620-8.
3. Gil J and Esteban M. Induction of apoptosis by the dsRNA-dependent protein kinase (PKR): Mechanism of action. Apoptosis 2000; 5:107-14.
4. Gil J, Garcia MA, and Esteban M. Caspase 9 activation by the dsRNA-dependent protein kinase, PKR: Molecular mechanism and relevance. FEBS Lett. 2002; 19:3665-74.
Related Products
PKR (CT) Blocking Peptide (Leinco Prod. No.: P230)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A431 Whole Cell Lysate (Human Epidermoid Carcinoma) (Leinco Prod. No.: A180)
Anti-Human PKR (NT) (Leinco Prod. No.: P229)
PKR (NT) Blocking Peptide (Leinco Prod. No.: P231)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
