产品编号:R1233
产品描述:Anti-Human Rheb
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Ras Homolog Enriched in Brain (Rheb)
Purified No Carrier Protein
Polyclonal Antibody
Prod. No.: R1233
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Ras Homolog Enriched in Brain (Rheb) recognizes an epitope in the intermediate domain of Human, Mouse and Rat Rheb. This polyclonal antibody was purified using affinity chromatography.
Background:
Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure.1 While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway.2 Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway.3-4 TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.3
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
Ras Homolog Enriched in Brain (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: R1239)
Blocking Peptide
Ras Homolog Enriched in Brain (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: R1239)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat Rheb by Western blot analysis at a concentration of 1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Mouse Heart Tissue Lysate (Leinco Prod. No.: M1013).
References
1. Yamagata, K. et al. (1994) J. Biol. Chem. 269:16333
2. Yee, W. M. et al. (1997) Mol. Cell. Biol. 17:921
3. Inoki, K. et al. (2003) Genes Dev. 17:1829
4. Stocker, H. et al. (2003) Nat. Cell Biol. 5:559
Related Products
Ras Homolog Enriched in Brain (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: R1239)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Mouse Heart Tissue Lysate (Leinco Prod. No.: M1013)
Anti-Human Ras Homolog Enriched in Brain (NT) (Rheb) (Leinco Prod. No.: R1234)
Ras Homolog Enriched in Brain (NT) Blocking Peptide (Leinco Prod. No.: R1238)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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