产品编号:R1235
产品描述:Anti-Human RPS6K1
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human Ribosomal Protein S6 Kinase 1 (RPS6K1)
Purified No Carrier Protein
(S6K, p70 S6 Kinase, p70-S6K)
Polyclonal Antibody
Prod. No.: R1235
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human Ribosomal Protein S6 Kinase 1 (RPS6K1) recognizes Human RPS6K1. This polyclonal antibody was purified using affinity chromatography.
Background:
Ribosomal protein S6 kinase 1 (RPS6K1) is the best characterized effector of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors/1,2 Nutrients and growth factors stimulate a complex including TOR, raptor (regulatory associated protein of TOR), and GBL to phosphorylate RPS6K1 and the eukaryotic initiation factor 4E binding protein (4EBP1), leading to increased protein synthesis and cell growth.3 RPS6K1 is thought to desensitize tissues to insulin as mice deficient in RPS6K1 have been shown to be hypersensitive to insulin and impervious to obesity-induced insulin resistance seen in wild type obese mice.4
Known Species Cross Reactivity: Hu+
Immunogen
Ribosomal Protein S6 Kinase 1 Blocking Peptide (Leinco Prod. No.: R1240)
Blocking Peptide
Ribosomal Protein S6 Kinase 1 Blocking Peptide (Leinco Prod. No.: R1240)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human RPS6K1 by Western blot analysis at a concentration of 5.0-10.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Jurkat Whole Cell Lysate (Leinco Prod. No.: J103).
References
1. Burnett, P. E. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1432
2. Inoki, K. et al. (2005) Mol. Biol. Rev. 69:79
3. Manning, B. D. (2004) J. Cell Biol. 167:399
4. Um, S. H. et al. (2004) Nature 431:200
Related Products
Ribosomal Protein S6 Kinase 1 Blocking Peptide (Leinco Prod. No.: R1240)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Jurkat Whole Cell Lysate (Leinco Prod. No.: J103)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
