产品编号:R999

产品描述:Anti-Human RICK(CT)

反应种属:

实验方法:

标记:

规格:0.5mg

供应商:Leinco

价格(RMB):16095.00

订购:

说明书:

Rabbit Anti-Human RICK
Purified No Carrier Protein
(RIP2, CARDIAK)
Polyclonal Antibody

Prod. No.: R999
Host:         Rabbit
Conc.:      0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage:      2-8°C

Description
Specificity:
Rabbit Anti-Human RICK recognizes an epitope near the C-terminus of Human RICK. This polyclonal antibody was purified using affinity chromatography.

Background:
Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively.1-3 RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues.1

Known Species Cross Reactivity: Hu+
Immunogen
RICK (CT) Blocking Peptide (Leinco Prod. No.: R1006)
Blocking Peptide
RICK (CT) Blocking Peptide (Leinco Prod. No.: R1006)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human RICK by Western blot analysis at a dilution of 1:500 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are K562 Whole Cell Lysate (Leinco Prod. No.: K128) and 3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183).

References
1. Inohara, N. et al. (1998) J. Biol. Chem. 273:12996
2. McCarthy, J. V. et al. J. Biol. Chem. 273:16968
3. Thome, M. et al. (1998) Curr. Biol. 8:885
Related Products
RICK (CT) Blocking Peptide (Leinco Prod. No.: R1006)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
3T3 Balb Whole Cell Lysate (Leinco Prod. No.: A183)
Anti-Human RICK (NT) (Leinco Prod. No.: R1000)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.