产品编号:R1000
产品描述:Anti-Human RICK(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human RICK
Purified No Carrier Protein
(RIP2, CARDIAK)
Polyclonal Antibody
Prod. No.: R1000
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human RICK recognizes an epitope near the N-terminus of Human, Mouse and Rat RICK. This polyclonal antibody was purified using affinity chromatography.
Background:
Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively.1-3 RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1b. The messenger RNA of RICK is expressed in multiple human tissues.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
RICK (NT) Blocking Peptide (Leinco Prod. No.: R1007)
Blocking Peptide
RICK (NT) Blocking Peptide (Leinco Prod. No.: R1007)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat RICK by Western blot analysis at a dilution of 1:1000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive controls for Western blotting are A431 Whole Cell Lysate (Human Epidermoid Carcinoma) (Leinco Prod. No.: A180) and K562 Whole Cell Lysate (Leinco Prod. No.: K128).
References
1. Inohara, N. et al. (1998) J. Biol. Chem. 273:12996
2. McCarthy, J. V. et al. J. Biol. Chem. 273:16968
3. Thome, M. et al. (1998) Curr. Biol. 8:885
Related Products
RICK (NT) Blocking Peptide (Leinco Prod. No.: R1007)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
A431 Whole Cell Lysate (Human Epidermoid Carcinoma) (Leinco Prod. No.: A180)
K562 Whole Cell Lysate (Leinco Prod. No.: K128)
Anti-Human RICK (CT) (Leinco Prod. No.: R999)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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