产品编号:R1245
产品描述:Anti-Human RP105(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human RP105
Purified No Carrier Protein
(Radioprotective 105, Lymphocyte Antigen 64, CD180 Antigen Precursor)
Polyclonal Antibody
Prod. No.: R1245
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human RP105 recognizes an epitope near the C-terminus of human and mouse RP105. This polyclonal antibody was purified using affinity chromatography.
Background:
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1,2). The signaling of these TLRs is kept under tight control by the expression of endogenous inhibiting proteins. One such protein is RP105, a recently identified homolog to TLR4 that, with MD-1, interacts with and inhibits the TLR4/MD-2 signaling pathway (3). It has also been suggested that the P105/MD-1 complex influences antibody production mediated by both TLR4/MD-2 and TLR2 receptor complexes (4). Despite its predicted molecular weight, RP105 often migrates at 95 – 105 kDa.
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
RP105 (CT) Blocking Peptide (Leinco Prod. No.: R1248)
Blocking Peptide
RP105 (CT) Blocking Peptide (Leinco Prod. No.: R1248)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), WB
Western Blotting: This antibody can be used to detect human and mouse RP105 by Western blot analysis at a concentration of 0.5 - 1 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Human Spleen Tissue Lysate (Leinco Prod. No.: H1410).
References
1. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
2. Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
3. Divanovic S, Trompette A, Atabani SF, et al. Inhibition of TLR-4/MD-2 signaling by RP105/MD-1. J. Endotoxin Res. 2005; 11:363-8.
4. Nagai Y, Kobayashi T, Motoi Y, et al. The radioprotective 105/MD-1 complex links TLR2 and TLR4/MD-2 in antibody response to microbial membranes. J. Immunol. 2005; 174:7043-9.
Related Products
RP105 (CT) Blocking Peptide (Leinco Prod. No.: R1248)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Human Spleen Tissue Lysate (Leinco Prod. No.: H1410)
Anti-Human RP105 (Intermediate Domain) (Leinco Prod. No.: R1246)
Anti-Human RP105 (NT) (Leinco Prod. No.: R1247)
RP105 (Intermediate Domain) Blocking Peptide (Leinco Prod. No.: R1249)
RP105 (NT) Blocking Peptide (Leinco Prod. No.: R1250)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
