产品编号:S555
产品描述:Anti-Human SARM(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human SARM
Purified No Carrier Protein
(Sterile Alpha and TIR Motif Containing 1 )
Polyclonal Antibody
Prod. No.: S555
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1.0 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human SARM recognizes an epitope near the C-terminus of human and mouse SARM. This polyclonal antibody was purified using affinity chromatography.
Background:
Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). SARM (SAM and ARM-containing protein), along with other molecules such as TIRP, TRIF, TIRAP, and MyD88, is thought to serve as an adaptor protein for the TLRs that allows for the activation of downstream kinases and NF-kB, and ultimately the expression of proteins involved in host defense (4,5). While SARM has not been conclusively shown to associate directly with TLRs, the presence of a Toll-interluekin-1 (TIR) domain in SARM is consistent with a role as a signaling molecule (4,5).
Known Species Cross Reactivity: Ms+, Hu+
Immunogen
SARM (CT) Blocking Peptide (Leinco Prod. No.: S558)
Blocking Peptide
SARM (CT) Blocking Peptide (Leinco Prod. No.: S558)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (Frozen), WB, In vivo Depletion
Western Blotting: This antibody can be used to detect human and mouse SARM by Western blot analysis at a concentration of 0.5 - 2 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Daudi Whole Cell Lysate (Leinco Prod. No.: D310).
References
1. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
2. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
3. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
4. O’Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.
Related Products
SARM (CT) Blocking Peptide (Leinco Prod. No.: S558)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Daudi Whole Cell Lysate (Leinco Prod. No.: D310)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
