产品编号:S520
产品描述:Anti-Human SHP2(NT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):17925.00
订购:
说明书:
Rabbit Anti-Human SHP2
Purified No Carrier Protein
(Tyrosine-Protein Phosphatase Non-receptor Type 11, PTP-2C)
Polyclonal Antibody
Prod. No.: S520
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human SHP2 recognizes an epitope near the N-terminus of Human, Mouse and Rat SHP2. This polyclonal antibody was purified using affinity chromatography.
Background:
Src homology-2 domain containing protein (SHP2) is a member of the protein tyrosine phosphatase (PTP) family, a protein family that contains signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. SHP2 contains two tandem Src homology-2 (SH2) domains, which function as phosphotyrosine binding domains either directly or through scaffolding intermediates such as the insulin-receptor substrate 1 (IRS-1). These SH2 domains mediate the interaction of SHP2 with its substrates, allowing SHP2 to dephosphorylate proteins that inhibit signaling kinases such as ERK1 and AKT. SHP2 is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Recent experiments have shown SHP2 plays a significant role in hepatoprotection and liver regeneration.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
SHP2 (NT) Blocking Peptide (Leinco Prod. No.: S527)
Blocking Peptide
SHP2 (NT) Blocking Peptide (Leinco Prod. No.: S527)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
WB
Western Blotting: This antibody can be used to detect Human, Mouse and Rat SHP2 by Western blot analysis at a concentration of 0.5-1.0 µg/ml when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is Mouse Skeletal Muscle Tissue Lysate (Leinco Prod. No.: M1019).
References
1. Yu, Z. et al. (1997) J. Biol. Chem. 272:7519
2. Ostman, A. et al. (2006) Nat. Rev. Cancer 6:307
3. Bard-Chapeau, E. A. et al. (2006) Mol. Cell. Biol. 26:4664
Related Products
SHP2 (NT) Blocking Peptide (Leinco Prod. No.: S527)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
Mouse Skeletal Muscle Tissue Lysate (Leinco Prod. No.: M1019)
Anti-Human SHP2 (CT) (Leinco Prod. No.: S519)
SHP2 (CT) Blocking Peptide (Leinco Prod. No.: S526)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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