产品编号:S487
产品描述:Anti-Human SIRPα(CT)
反应种属:
实验方法:
标记:
规格:0.5mg
供应商:Leinco
价格(RMB):16095.00
订购:
说明书:
Rabbit Anti-Human SIRPα
Purified No Carrier Protein
(SHPS-1, MyD-1, BIT, p84)
Polyclonal Antibody
Prod. No.: S487
Host: Rabbit
Conc.: 0.5 mg/ml
Pkg. Size: 1 mg, 0.1 mg, 0.5 mg
Storage: 2-8°C
Description
Specificity:
Rabbit Anti-Human SIRPα recognizes an epitope near the C-terminus of Human, Mouse and Rat SIRPα. This polyclonal antibody was purified using affinity chromatography.
Background:
Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPα, SHPS-1, MyD-1, BIT and p84. SIRPα is a new gene family containing at least fifteen members. SIRPα is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPα has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
Known Species Cross Reactivity: Ms+, Hu+, Rt+
Immunogen
SIRPα (CT) Blocking Peptide (Leinco Prod. No.: S529)
Blocking Peptide
SIRPα (CT) Blocking Peptide (Leinco Prod. No.: S529)
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Stability
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
IHC (NBF/Par.), WB
Western Blotting: This antibody can be used to detect Human SIRPα by Western blot analysis at a dilution of 1:1000-1:2000 when used in conjunction with compatible secondary reagents, such as Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190), under either reducing or non-reducing conditions. The positive control for Western blotting is THP-1 Whole Cell Lysate (Leinco Prod. No.: T206).
References
1. Kharitonenkov, A. et al. (1997) Nature 386:181
2. Fujioka, Y. et al. (1996) Mol. Cell Biol. 16:6887
3. Yamao, T. et al. (1997) Biochem. Biophys. Res. Commun. 231:61
4. Brooke, G. P. et al. (1998) Eur. J. Immunol. 28:1
Related Products
SIRPα (CT) Blocking Peptide (Leinco Prod. No.: S529)
Goat Anti-Rabbit IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: R1190)
THP-1 Whole Cell Lysate (Leinco Prod. No.: T206)
SIRPα (CT) Blocking Peptide (Leinco Prod. No.: S489)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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